Transformation of ecoli with pglo dan prochaska and jeff winslow talawanda/butler tech download presentation transformation of ecoli with pglo loading in 2 seconds observe and record actual plate data draw conclusions from the experiment and list possible sources of error. The bacterium, e coli, is the ideal host for transformation because it is a small, single-celled organism that reproduces quickly, so its transformation use a plasmid vector to transform bacteria with genes for green fluorescent protein (gfp) and antibiotic resistance in a controlled experiment. Genetic transformation is an extremely useful process for science, being used by pharmaceutical companies to produce large quantities of antibiotics or for our experiment, the group is tasked with transforming a harmless strain of e coli bacteria with a plasmid dna that we are given called pglo. Although the e coli strain used in these experiments has been rendered non-pathogenic, it student activity: transformation of the bacterium e coli using a gene for green fluorescent protein on plate 2 the transformed bacteria can still grow to form a lawn since there is no antibiotic present to.
Pglo transformation of e coli a procedure known as genetic transformation has been conducted on the bacterium escherichia coli (e coli), strain hbioi :k-12 this strain is unicellular, reproduces every 20 minutes in optimum conditions, does not make people sick, and it cannot survive outside the. Report abuse transcript of bacterial transformation: manipulation of e coli discussion looking back at our results, i think the experiment was in the petri dish with +pglo/lb/amp which took in the pglo gene shown growth but not a large amount meaning the bacteria that didn't take in the. Connor lauffenburger 3/17/13 pglo transformation lab report i introduction the purpose of this experiment was to show the genetic transformation of e coli bacteria with a plasmid that codes for green fluorescent protein (gfp) and contains a gene regulatory system that confers ampicillin. Transformation of the bacterium e coli using a gene for green fluorescent protein background in molecular biology, transformation refers to arac ori pglo bla gfp biotechnology explorer bacterial transformation the pglo system catalog number 166-0003-edu wwwbio-radcom for technical.
Practical steps to get ecoli to express the glowing jellyfish gene using the bio rad pglo kit. Transformation of ecoli with pglow pglo tm bacterial transformation transformation - effingham county schools protein is produced - blocking and unblocking is how bacteria make certain proteins certain times - example: lactose some steps • transformation solution - ca2.
Escherichia coli or e coli is a gram negative, bacillus, bacteria e coli makes a great bacterial candidate for the bacterial transformation because the pglo plasmid also contains a special gene regulation system, or operon, that can be used to control expression of the florescent protein in the. Bacteria strains of e coli were genetically transformed with a plasmid to carry ampicillin resistance 250 microliters of a cacl2 transformation solution were this experiment is aimed at exploring the transformation of ecoli bacteria with a genetically engineered dna called pglo plasmids. Abstract: this experiment focuses on genetic engineering and transformation of bacteria the goal of the experiment is to transform e coli with pglo plasmid, which carries a gene for ampicillin resistance, and determine the transformation efficiency.
Of bacteria lab (to transform e coli bacteria with a gene encoding green fluorescent protein -gfp using pglo plasmid along with lb agar and ampicillin), 1 what new gene (s) were you looking for in the transformed e coli 2 why do you expect colonies on the ampicillin agar to fluoresce. This experiment deciphered the effects that gfp had on the bacteria e coli with the vector pglo there were four different plates used to determine the results of this experiment displayed a genetic transformation where e coli took up the gfp gene from a jellyfish with all of the correct material. Presentation on theme: genetic transformation of e coli bacteria— presentation transcript 4 target organism: e coli prokaryote with circular loop of dna plasmids are small circles of bonus dna pglo plasmids cell wall gfp bacterial chromosomal dna.
Using pglo to transform bacteria, students can actually observe gene expression in real time following the transformation with bio-rad's gfp purification the focus here is not so much on the answer or result, but rather on how the result was obtained and how it can be substantiated by careful. Experiment 11 laboratory to biologieiii: diversity of microorganisms tuesday group d transformation of pglo into escherichia coli author the +dna culture: the lb+amp plate allows only the transformed bacteria to grow, because they received the ampicillin-resistence-gene. E coli transformation with pglo abstract to conclude, this experiment showed that arabinose (ara) and uv light should be within a bacteria's environment so that you can visit a fluorescent green glow in the bacteria's colonies.